| Data | Casa | Visitante | Resultado |
|---|---|---|---|
| 28-10-25 18:00 | CF Ourense | Real Oviedo | 4:2a.p. |
| 28-10-25 18:00 | Atletico Tordesillas | Burgos CF | 1:2a.p. |
| 28-10-25 18:00 | CE Constància | FC Girona | 2:3a.p. |
| 28-10-25 18:30 | SD Logroñés | Racing Santander | 0:4 |
| 28-10-25 18:30 | UP Langreo | Racing de Ferrol | 3:4a.pen. |
| 28-10-25 18:30 | UCAM Múrcia | FC Cádiz | 1:3 |
| 28-10-25 19:00 | CD Guadalajara | CP Cacereño | 2:1 |
| 28-10-25 19:00 | CF Rayo Majadahonda | CF Talavera de la Reina | 1:4 |
| 28-10-25 19:00 | UD San Fernando | Albacete Balompie | 0:3 |
| 28-10-25 19:00 | Inter de Valdemoro | FC Getafe | 0:11 |
| 28-10-25 19:00 | CD Roda | FC Granada | 1:5 |
| 28-10-25 19:00 | CD Tropezon | CyD Leonesa | 1:3 |
| 28-10-25 19:00 | UD Maracena | FC Valencia | 0:5 |
| 28-10-25 19:30 | Utebo | SD Huesca | 0:3 |
| 28-10-25 19:30 | RSD Alcalá | CD Teneriffa | 0:4 |
| 28-10-25 20:00 | CD Extremadura | UD Las Palmas | 3:1 |
| 28-10-25 20:00 | SD Negreira | Real Sociedad | 0:3 |
| 28-10-25 20:00 | CD Toledo | FC Sevilla | 1:4 |
| 29-10-25 17:00 | UD Poblense | CD Sabadell | 1:2a.p. |
| 29-10-25 18:00 | CD Ebro | SD Tarazona | 5:4a.pen. |
| 29-10-25 18:00 | Union Mutilvera | Real Zaragoza | 1:3 |
| 29-10-25 18:00 | CD Sant Jordi | CA Osasuna | 0:5 |
| 29-10-25 18:00 | Los Garres | FC Elche | 0:4 |
| 29-10-25 18:30 | La Unión Atlético CF | AD Ceuta FC | 0:2 |
| 29-10-25 19:00 | CD Artistico Navalcarnero | Mérida AD | 2:1 |
| 29-10-25 19:00 | CD Azuaga | CD Leganés | 1:4 |
| 29-10-25 19:00 | Náxara CD | SD Eibar | 2:4 |
| 29-10-25 19:00 | Atletico Astorga | CD Mirandés | 4:5a.pen. |
| 29-10-25 19:00 | CD Numancia | Arenas Club de Getxo | 3:0 |
| 29-10-25 19:00 | Lorca Deportiva CF | UD Almeria | 0:2 |
| 29-10-25 19:00 | UE Sant Andreu | CD Teruel | 2:1a.p. |
| 29-10-25 19:00 | Caudal Deportivo | Sporting Gijón | 0:1 |
| 29-10-25 19:00 | Atlètic Sant Just | RCD Mallorca | 0:2 |
| 29-10-25 19:00 | Torrent CF | Juventud Torremolinos CF | 3:1 |
| 29-10-25 19:00 | CD Cieza | Cordóba CF | 1:0 |
| 29-10-25 19:15 | Club Portugalete | Real Valladolid | 1:0 |
| 29-10-25 19:30 | CF Reús Deportiu | CE Europa | 6:5a.pen. |
| 29-10-25 19:30 | UD Ourense | CF Pontevedra | 1:4a.pen. |
| 29-10-25 19:30 | Puente Genil CF | FC Cartagena | 0:1 |
| 29-10-25 20:00 | CD Quintanar del Rey | UD Ibiza Eivissa | 5:4a.pen. |
| 29-10-25 20:00 | Real Jaén C.F. | CD Eldense | 1:3 |
| 29-10-25 20:00 | CF Lucena | FC Villarreal | 0:6 |
| 29-10-25 20:00 | CD Yuncos | Rayo Vallecano | 1:6 |
| 30-10-25 18:00 | Orihuela CF | UD Levante | 3:4 |
| 30-10-25 18:00 | Puerto de Vega CF | Celta Vigo | 0:2 |
| 30-10-25 18:30 | Valle de Egues | Andorra CF | 1:5 |
| 30-10-25 19:00 | Atletico Baleares | Gimnástic de Tarragona | 2:0 |
| 30-10-25 19:00 | UD Logroñés | SD Ponferradina | 1:3 |
| 30-10-25 19:00 | Real Ávila CF | Real Avilés | 1:0a.p. |
| 30-10-25 19:00 | Arenas Club de Getxo | Deportivo Alavés | 0:7 |
| 30-10-25 20:00 | Real Murcia C. F. | Antequera CF | 1:1a.p. |
| 30-10-25 20:00 | CD Estepona | FC Málaga | 1:3 |
| 30-10-25 20:00 | CA Antoniano | CD Castellón | 1:0 |
| 30-10-25 20:00 | UD Samano | Deportivo La Coruña | 1:5 |
| 30-10-25 20:00 | Club Lleida Esportiu | Espanyol Barcelona | 1:2 |
| 30-10-25 20:00 | Atletico Palma del Rio | Real Betis Sevilla | 1:7 |